Cosmetic efficacy claim studies| Eviogen Discovery

Assay	Method details	Timeline
Cytotoxicity	MTT and CTG, in keratinocytes (HaCat), normal dermal fibroblast and melanoma cells (murine B16/F10) separately or co-culture	2-3 weeks
Anti aging effects (Skin regeneration and anti-wrinkle)	Upregulation of Collagen-I synthesis and downregulation of hyaluronidase, elastase, collagenase-14 and MMP-11
Melanin formation	Melanin inhibitory activity in B16/F10 murine melanoma cells
Cell migration and healing effects	Microscopy
Phototoxicity	In Vitro 3T3 NRU Phototoxicity Test
Skin whitening	Tyrosinase inhibitory assay (cell-free)
In-vitro skin irritation and corrosion test	Reconstructed Human Epidermis (RHE) (According to OECD guidelines)	5-6 weeks

• Custom in-vitro assay development
• Human volunteer studies
• Animal studies

Cosmetic efficacy claim studies